The Americana Collection offers more than 4,000 diverse American imprints and manuscripts. The focus of the collection is on Colonial America, the Revolutionary War Era, and the early Republic, but the breadth of the collection spans five centuries. The DAR treasury includes diaries and letters; military papers; governmental papers; birth, baptismal and marriage records; autographs; newspapers; maps; legal and court records; and music. Visitors to the Americana Room can view the collection's holdings arranged in exhibits which change periodically to reflect different themes. Past exhibits have captured the essence of the American Revolution, illustrated schooling in America, and showcased Signers of the United States Constitution.

The NSDAR Archives serves as the repository for NSDAR records which are no longer administratively useful but which have sufficient historical or other value to warrant their continued preservation. The NSDAR Archives houses the non-current permanent records created in the course of business by the National Society—items such as correspondence, meeting minutes, project files, photographs and the like.